Construction of mesoporous silica as an inorganic nano-drug carrier and its bioactivity

doi:10.3969/j.issn.2095-4344.0935

Abstract

Abstract:

BACKGROUND: Numerous studies have addressed the construction of stimulating response drug transport systems based on the mesoporous silica platform; however, drug leakage is still inevitable in the control cycle.

OBJECTIVE: To study the preparation and bioactivity of mesoporous silica (MS@FcAA/P@CD@RGD).

METHODS: MCM-41 mesoporous silica nanoparticles were used as a release carrier of intracellular controlled drugs. In the channel, ferrocene and fluorescent probes were packaged, and reoccupied using beta-cyclodextrin, and integrin inhibitors RGD were used as targeted groups to synthesize mesoporous silica nanoparticles (MS@FcAA/P@CD@RGD). MTT assay was performed on human cervical cancer cell line HeLa and human breast cancer cell MCF-7 as target cells and control cells, respectively, to evaluate the cytotoxicity of mesoporous silica nano-drug carriers with different mass concentrations. The passaged HeLa cells were cultured in three groups: a medium containing phorbol ester (induced a large amount of H₂O₂ in cells)+MS@FcAA/P@CD@RGD, a medium containing dimethyl sulfoxide (removal of intracellular H₂O₂)+MS@FcAA/P@CD@RGD, or a medium containing MS@FcAA/P@CD@RGD. Then the particle size was measured by Laser scanning confocal microscope, and the response of the nano-drug delivery system to intracellular H₂O₂ was assessed at 3 days after culture.

RESULTS AND CONCLUSION: When the mesoporous silica nanoparticles were within the concentration of 10-100 mg/L, over 85 % of the cells were alive. Compared with the cells cultured in the medium containing MS@FcAA/P@CD@RGD, the fluorescence intensity of HeLa cells with phorbol ester were significantly increased, while the fluorescence intensity of HeLa cells with dimethyl sulfoxide were significantly reduced. To conclude, mesoporous silica nanoparticles have a low cytotoxicity, and have certain response to endogenous hydrogen peroxide.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Silicon Dioxide, Nanocomposites, Drug Toxicity, Tissue Engineering

CLC Number:

R318

Liu Ying, Shi Wei, Gong Rui, Zhu Hong-ming. Construction of mesoporous silica as an inorganic nano-drug carrier and its bioactivity[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(26): 4144-4149.

Figures/Tables 3

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